Analysing cells using SPR

fdtd

#1

Hi,

I am new to SPR related studies. I would like to study SPR angle for single cell trapped in a microdroplet. The diameter of droplet containing a cell is about 400um and cell size is about 20um. Pls let me know the following.

Should I model and define the droplets and cells along with its RI on the Gold layer?

What wavelength sizes are recommended to be operated “nm” or “um”?


#2

Depending on the wavelength range you are interested in simulating, the full structure with 400 um diameter droplet might be difficult to simulate using a 3D FDTD simulation due to it’s large size relative to optical wavelengths.

You might want to first start with a smaller region inside the droplet where there is a gold substrate and the cell inside a background medium with refractive index of water. This way you could still get the resonance frequencies for different incident angles of light.